ࡱ > > *7 bjbj 4 */ <" <" <" <" <" P" P" P" P" 4 " P" * x# $$ $$ $$ $$ #% #% #% I* K* K* K* K* K* K* $ p, / T o* <" #% #% #% #% #% o* <" <" $$ $$ * m) m) m) #% : <" $$ <" $$ I* m) #% I* m) m) m) $$ f P" ]( m) 5* * 0 * m) f/ ( f/ m) m) f/ <" ) #% #% m) #% #% #% #% #% o* o* m) #% #% #% * #% #% #% #% f/ #% #% #% #% #% #% #% #% #% ! : Supplementary material related to the paper entitled Selection of reference genes for quantitative real-time PCR normalization in adipose tissue, muscle, liver and mammary gland from ruminants by M. Bonnet, L. Bernard, S. Bes and C. Leroux
Figure captions
Supplementary Figure 1. Representative electropherograms for total RNA extracted from two samples of adipose tissue, muscle, mammary gland or liver. From this analysis, the presence of two peaks corresponding to the 18S and 28S RNA, as well as the RNA integrity number (RIN) between 8 and 10 ensured the high RNA quality. The x axis is the time in seconds (s).
Supplementary Figure 2. Specificity of real-time PCR amplification shown by a dissociation curve with a single pick that was obtained from two technical replicates of two bovine and two caprine samples (except for TOP2B, which were only amplified in bovine). The negative control (PCR amplification from water) did not produce an amplicon and, thus, a dissociation curve.
Supplementary Table 1 Description of experiments in which the provided bovine and caprine tissues were used to select reference genes for RT- qPCR data normalisation.
TissueBreedAge (months)Physiological
statusAnimal numberVariation factorSourceBovine speciesMammary glandHolstein36Late-lactating 3Hay-based dietUnpublished dataHolstein36Late-lactating3Concentrate-based diet + sunflower oil (6.1 %)1Unpublished dataAdipose tissueLimousin28Growing steers4Low adiposity, high concentrate-based diet ADDIN EN.CITE ADDIN EN.CITE.DATA (Bonnet et al., 2007)Angus28Growing steers4High Adiposity, high concentrate-based diet ADDIN EN.CITE ADDIN EN.CITE.DATA (Bonnet et al., 2007)LiverBlond dAquitaine19Growing steers4Concentrate-based diet(Gruffat et al.)Blond dAquitaine19Growing steers4Concentrate-based diet + extruded linseed (4.9 %)(Gruffat et al.)MuscleNormande57Dry cows4Concentrate-based diet ADDIN EN.CITE Bonnet201157835783578310Bonnet, M.Cassar-Malek, I.Bauchart, D.Chilliard, Y.Jurie, C.Thomas, A.Normand, J.TALL-AGL AMUVILipid supplementation and basal diet influence oxidative and lipogenic metabolism in muscle and adipose tissues in cattleProceedings of the Eight International Symposium on the Nutrition of Herbivores53922011Eighth International Symposium on the Nutrition of Herbivores, 6-9 September 2011, Aberystwyth, Wales UK (r�m� affiche)(Bonnet et al., 2011)Normande57Dry cows4Concentrate-based diet + palmitostearin (5.9 %) ADDIN EN.CITE Bonnet201157835783578310Bonnet, M.Cassar-Malek, I.Bauchart, D.Chilliard, Y.Jurie, C.Thomas, A.Normand, J.TALL-AGL AMUVILipid supplementation and basal diet influence oxidative and lipogenic metabolism in muscle and adipose tissues in cattleProceedings of the Eight International Symposium on the Nutrition of Herbivores53922011Eighth International Symposium on the Nutrition of Herbivores, 6-9 September 2011, Aberystwyth, Wales UK (r�m� affiche)(Bonnet et al., 2011)Caprine speciesMammary glandAlpine42Late-lactating4
Natural grassland hay dietUnpublished data42Late-lactating4Natural grassland hay diet + extruded linseed + 1.7% fish oil (6.9 %)Unpublished dataAdipose TissueAlpine54Mid-lactating4standard diet ad libitum ADDIN EN.CITE Faulconnier201157545754575417Faulconnier, Y.Chilliard, Y.Torbati, M. B.Leroux, C.Unite de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Theix, Saint Genes-Champanelle, France.The transcriptomic profiles of adipose tissues are modified by feed deprivation in lactating goatsComp Biochem Physiol Part D Genomics ProteomicsComp Biochem Physiol Part D Genomics Proteomics139-49622011/01/25Adipose Tissue/cytology/*metabolismAnimal FeedAnimalsBody WeightFasting/blood/*metabolismFemale*Gene Expression ProfilingGene Expression RegulationGoats/blood/*genetics/metabolismInsulin/blood*LactationLeptin/bloodLipid Metabolism2011Jun1878-0407 (Electronic)
1744-117X (Linking)21256818http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21256818S1744-117X(10)00106-1 [pii]
10.1016/j.cbd.2010.12.002eng(Faulconnier et al., 2011)54Mid-lactating448-hour fasting ADDIN EN.CITE Faulconnier201157545754575417Faulconnier, Y.Chilliard, Y.Torbati, M. B.Leroux, C.Unite de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Theix, Saint Genes-Champanelle, France.The transcriptomic profiles of adipose tissues are modified by feed deprivation in lactating goatsComp Biochem Physiol Part D Genomics ProteomicsComp Biochem Physiol Part D Genomics Proteomics139-49622011/01/25Adipose Tissue/cytology/*metabolismAnimal FeedAnimalsBody WeightFasting/blood/*metabolismFemale*Gene Expression ProfilingGene Expression RegulationGoats/blood/*genetics/metabolismInsulin/blood*LactationLeptin/bloodLipid Metabolism2011Jun1878-0407 (Electronic)
1744-117X (Linking)21256818http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21256818S1744-117X(10)00106-1 [pii]
10.1016/j.cbd.2010.12.002eng(Faulconnier et al., 2011)LiverAlpine42Late-lactating4Hay-based diet + sunflower oil and SDS (8.9 % ) ADDIN EN.CITE Bernard201257465746574617Bernard, L.Leroux, C.Rouel, J.Bonnet, M.Chilliard, Y.INRA, UR 1213 Herbivores, Site de Theix, F-63122 Saint-Genes-Champanelle, France.Effect of the level and type of starchy concentrate on tissue lipid metabolism, gene expression and milk fatty acid secretion in Alpine goats receiving a diet rich in sunflower-seed oilBritish Journal of NutritionBritish Journal of Nutrition114711591072011/08/312012Aug 301475-2662 (Electronic)
0007-1145 (Linking)21875448http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21875448S0007114511004181 [pii]
10.1017/S0007114511004181Eng(Bernard et al., 2012)42Late-lactating4Hay-based diet + sunflower oil and RDS (7.4 %) ADDIN EN.CITE Bernard201257465746574617Bernard, L.Leroux, C.Rouel, J.Bonnet, M.Chilliard, Y.INRA, UR 1213 Herbivores, Site de Theix, F-63122 Saint-Genes-Champanelle, France.Effect of the level and type of starchy concentrate on tissue lipid metabolism, gene expression and milk fatty acid secretion in Alpine goats receiving a diet rich in sunflower-seed oilBritish Journal of NutritionBritish Journal of Nutrition114711591072011/08/312012Aug 301475-2662 (Electronic)
0007-1145 (Linking)21875448http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=21875448S0007114511004181 [pii]
10.1017/S0007114511004181Eng(Bernard et al., 2012)SDS refers to slowly degradable starch from corn grain; and RDS is rapidly degradable starch from flattened wheat. 1Percents refer to the % of ether extract in the dry matter.
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