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Table S1. Primer and probe sequences and conditions used for real-time RT-PCR.
Gene Encoded proteinAccession no.Nucleotide sequence (5 3)T (C)SourceCD36Platelet glycoprotein 4X91503F: ACA gAT gTg gCT TgA gCg Tg58 ADDIN EN.CITE Ollier200716511651165117Ollier, S.Robert-Granie, C.Bernard, L.Chilliard, Y.Leroux, C.Unite de Recherches sur les Herbivores, Institut National de la Recherche Agronomique, Theix, 63122 Saint Genes-Champanelle, France.Mammary transcriptome analysis of food-deprived lactating goats highlights genes involved in milk secretion and programmed cell deathJ NutrJournal of NutritionJ. Nutr.J Nutr560-713732007/02/22Animal Nutritional Physiological PhenomenaAnimalsApoptosis/*geneticsFemaleFood Deprivation/*physiologyGoatsLactation/geneticsMammary Glands, Animal/*cytology/*physiology/secretionMilk/*secretionOligonucleotide Array Sequence AnalysisTranscription, Genetic2007Mar0022-3166 (Print)
0022-3166 (Linking)17311940http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17311940137/3/560 [pii]engBernard et al., 2012R: ACT ggg TCT gTg TTT TgC AggSLC27A6Solute carrier family 27 member 6HYPERLINK "http://www.ncbi.nlm.nih.gov/nuccore/155372064"NM_001101169F: ACC ACA gAg gTT ggT gCT ATC60This articleR: CAA CCA CAC CAT AgA CAT TTg CFASNFatty acid synthase DQ223929F: ACA GCC TCT TCC TGT TTG ACG60 ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707eng ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707engBernard et al., 2005 ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707engR: CTC TGC ACG ATC AGC TCG ACP: ATC TGG AGG CGC GTG TGG CAG CCFADS1Fatty acid desaturase 1NM_001083517F TTT GTG ATC GGT CAC CTG AA:58Cherfaoui et al., 2012R: TGG TGC TGG AAG TGC AAG TFADS2Fatty acid desaturase 2NM_001083444F: AAA GGG TGC CTC TGC CAA CT60Bionaz and Loor, 2008R: ACA CGT GCA GCAT GTT CACASCD1Stearoyl-CoA desaturaseAF325499F: TgC TgA CAA CTT ATC Tgg Atg C60 ADDIN EN.CITE Bonnet200012581258125817Bonnet, M. Leroux, C. Faulconnier, Y. Hocquette, J. F. Bocquier, F. Martin, P. Chilliard, Y.INRA, Unite de Recherches sur les Herbivores, Theix, 63122 Saint-Genes-Champanelle, France.Lipoprotein lipase activity and mRNA are up-regulated by refeeding in adipose tissue and cardiac muscle of sheepJ NutrJournal of NutritionJ. Nutr.J Nutr749-5613043' Untranslated Regions: geneticsAdipose Tissue: enzymology: *metabolism*Animal FeedAnimal NutritionAnimalsBase Sequence: geneticsBlotting, NorthernDNA, Complementary: geneticsFemaleGene ExpressionLipoprotein Lipase: *genetics: *metabolismMolecular Sequence DataPapillary Muscles: enzymology: *metabolismRNA, Messenger: *metabolismResearch Support, Non-U.S. Gov'tSheepUp-Regulation20002000 Apr0022-3166 (Print)10736325eng ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707eng ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707eng ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707engBernard et al. 2005R: Aag gAA TCC TgC AAA CAg CTAP: CCA GAG CCT GCA GAA GTG GCT GGT ATA ASCD5Stearoyl-CoA desaturaseNM_001112815F: AgA Agg ggA ggA AgC TTg AC58Lengi and Corl, 2007 ADDIN EN.CITE Bonnet200012581258125817Bonnet, M. Leroux, C. Faulconnier, Y. Hocquette, J. F. Bocquier, F. Martin, P. Chilliard, Y.INRA, Unite de Recherches sur les Herbivores, Theix, 63122 Saint-Genes-Champanelle, France.Lipoprotein lipase activity and mRNA are up-regulated by refeeding in adipose tissue and cardiac muscle of sheepJ NutrJournal of NutritionJ. Nutr.J Nutr749-5613043' Untranslated Regions: geneticsAdipose Tissue: enzymology: *metabolism*Animal FeedAnimal NutritionAnimalsBase Sequence: geneticsBlotting, NorthernDNA, Complementary: geneticsFemaleGene ExpressionLipoprotein Lipase: *genetics: *metabolismMolecular Sequence DataPapillary Muscles: enzymology: *metabolismRNA, Messenger: *metabolismResearch Support, Non-U.S. Gov'tSheepUp-Regulation20002000 Apr0022-3166 (Print)10736325engR: ggA ggC CAg gAA gTA ggA gTELOVL2Elongation of very long chain fatty acids like 2NM_001083517F: CTT CTT ACA TGA TGA CGC TGG T60Cherfaoui et al., 2012R: GGC TTT TTC CGG TAT GTC TGELOVL5Elongation of very long chain fatty acids like 5NM_001046597F: CTG AAT ACC TTC TCC ACT GGA GGA60Faulconnier et al., 2011R: GCT CCC TGT AAT ATG AAT GTG CAASREBF1Sterol regulatory element binding factor 1TC263657F: CCA gCT gAC AgC TCC ATT gA60Harvatine and Bauman, 2006R: TgC gCg CCC ACA Agg gALXRALiver X receptor alphaNM_001014861F: AAG ACG TCT GCG ATT GAG GT62Cherfaoui et al., 2012R: TCT CAC TTC CAG GGT TGT ACCPPARAPeroxisome proliferator-activated receptor alphaNM_001034036F: CGC GGA AAA GAG GCT TCT C60Cherfaoui et al., 2012R: TTC CAA GGT TTC CAT GGT GGPPARG1Peroxisome proliferator-activated receptor gamma 1F: CAg gTT TgA AAg AAg CCA CA 60Bonnet et al., 2007R: TTA Cgg AAA CgT CCC TCT Tg SP1Sp1 transcription factorF:ggC Agg TTC AgA Agg CAg Tgg T60This articleR: CAC TgT Tgg CAA gAC ggg CAAPPIAPeptidyl-prolyl cis-trans isomerase A or cyclophilin AXM_001252497F: ggA TTT Atg TgT CCA ggg Tgg TgA60 ADDIN EN.CITE Bonnet200712541254125417Bonnet, M. Faulconnier, Y. Leroux, C. Jurie, C. Cassar-Malek, I. Bauchart, D. Boulesteix, P. Pethick, D. Hocquette, J. F. Chilliard, Y.INRA, UR1213 Herbivores, Site de Theix, F-63122 St Genes Champanelle, France.Glucose-6-phosphate dehydrogenase and leptin are related to marbling differences among Limousin and Angus or Japanese Black x Angus steersJ Anim SciJournal of Animal ScienceJ. Anim. Sci.J Anim Sci2882-948511Adipose Tissue: *enzymology: metabolismAnimalsBody Composition: *physiologyCattle: genetics: *physiologyCrosses, GeneticGene Expression Regulation, EnzymologicGlucosephosphate Dehydrogenase: *blood: metabolismLeptin: *bloodMaleMeat: *standardsMuscle, Skeletal: enzymology: metabolismRNA, Messenger: metabolism20072007 Nov1525-3163 (Electronic)17591707engBonnet et al., 2000R: CAA gAT gCC Agg ACC TgT AtgP: TCT CCC CAT AgA Tgg ACT TgC CAC CAg TUXTUbiquitously-expressed transcriptHYPERLINK "http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucest&id=21789237"BQ676558F: TgT ggC CCT Tgg ATA Tgg TT60Kadegowda et al., 2009R: ggT TgT CgC TgA gCT CTg TgEIF3KEukaryotic translation initiation factor 3 subunit K NM_001034489F: CCA ggC CCA CCA AgA AgA A60Kadegowda et al., 2009R: TTA TAC CTT CCA ggA ggT CCA TgTT = PCR annealing temperature (C); F = forward primer; R = reverse primer; P = Taqman probe.
Table S2. Quantitative PCR performance of lipogenic genes, transcription factors and internal controls.
Cycle threshold (Ct)GeneMammary tissueLiverPerirenal adipose tissueOmental adipose tissueSlopeR2EfficiencyaCD3620-2124-2618-1917-18-3.690.9971.87SLC27A630-3624-2529-3428-34-3.700.9901.86FASN24-2630-3124-2724-28-3.530.9991.92FADS130-3225-2628-3029-31-3.810.9971.83FADS227-3017-1820-2221-23-3.570.9831.91SCD120-2223-2720-2420-25-3.540.9951.92SCD527-3029-3029-3028-30-3.380.9931.98ELOVL233-3524-2631-3231-32-3.610.9931.89ELOVL528-3124-2724-2523-25-3.730.9981.85SREBF120-2322-2319-2120-21-3.340.9961.99LXRA25-2720-2220-2121-3.650.9911.88PPARA30-3629-3429-3029-31-3.100.8352.10PPARG124-2625-2728-1917-18-3.440.9981.95SP124-2521-2320-2220-21-3.360.9971.98PPIA20-2120-2119-2019-21-3.510.9941.93UXT23-2523-2422-2322-23-3.350.9981.99EIF3K23-2523-2422-2322-23-3.500.9961.93R2 = determination of the standard curve.
a The efficiency was calculated as [10(-1 / Slope)]
Table S3. Effect of fish oil and additional starch on the fatty acid (FA) composition (g/100 g FA) of the mammary secretory tissue, liver, and omental and perirenal adipose tissues in dairy goats fed a diet containing sunflower-seed oil.
Mammary tissueLiverOmental adipose tissuePerirenal adipose tissueFA (g/100 g FA)SOSFOs.e.d.PSOSFOs.e.d.PSOSFOs.e.d.PSOSFOs.e.d.P8:00.150.170.0800.787ndnd--ndnd--ndnd--9:00.010.020.0070.319ndnd--ndnd--ndnd--10:02.432.400.4160.945<0.010.010.0040.2070.170.150.0250.5070.040.020.0050.01111:00.050.050.0110.537ndnd--ndnd--ndnd--12:02.372.050.3160.3430.020.030.0040.0300.090.110.0100.1410.060.060.0060.70513:00.150.140.0270.5930.010.020.0040.0970.020.020.0040.9990.020.020.0030.356iso-13:00.010.020.0040.537nd0.01--0.01<0.010.0040.5370.01<0.010.0040.53814:05.485.290.8900.8380.520.870.1770.0982.833.720.3430.0412.542.960.2680.158iso-14:00.080.070.0110.3170.030.040.0100.6280.120.090.0160.1310.090.070.0130.168c9-14:10.110.120.0260.928nd0.02--0.020.030.0050.3560.040.050.0150.52215:00.840.840.0620.9380.520.530.0670.8310.650.660.0730.8700.550.570.0670.830iso-15:00.210.230.0130.1460.210.260.0530.3380.330.260.0460.1650.300.230.0430.158c10-15:1 0.060.070.0090.4140.040.030.0050.168ndnd--ndnd--16:017.8720.560.5570.00311.4015.031.4660.04826.5630.581.8020.06724.2927.181.5640.113iso-16:00.260.260.0270.9290.210.260.0390.2440.420.330.0450.0950.380.290.0420.077c9-16:10.500.770.1170.0550.370.780.1330.0230.540.560.0830.8190.850.880.2730.937t9-16:1 + iso-17:01.021.090.1490.6781.431.610.2460.4750.770.530.0630.0080.730.610.0520.05017:00.670.760.0440.1161.021.090.1060.5071.681.560.1400.4051.701.630.1560.691anteiso-17:00.390.400.0370.6990.540.550.0960.9400.960.760.0730.0330.970.840.0830.161c10-17:1ndnd--0.030.050.0080.1070.010.010.0050.6220.020.170.1400.31818:010.687.710.8630.01423.9719.561.6140.03434.7232.391.1770.09530.6328.472.9540.492iso-18:00.050.060.0010.0010.080.110.0130.0400.170.120.0120.0060.200.170.0170.132c9-18:124.4921.272.5100.24710.0710.841.5520.63514.9516.260.9350.21021.5623.143.5730.673c11-18:10.690.950.0630.0060.600.820.0550.0070.590.500.0360.0500.600.560.0370.251c12-18:10.910.430.1520.0200.810.360.1540.0260.230.210.0460.7570.270.230.0350.326c13-18:10.110.140.0120.0220.090.100.0170.4900.060.050.0080.3560.080.080.0060.705c15-18:1 + 19:00.110.150.0130.0240.240.220.0430.6960.270.230.0290.2980.320.270.0460.342t4-18:10.030.020.0040.5370.030.040.0060.2280.030.030.0050.6200.030.040.0100.620t5-18:10.020.030.0080.2670.020.050.0070.0180.030.030.0040.9990.030.030.0050.390t6-,7-,8-18:10.360.480.0490.0550.230.300.0380.1190.320.290.0570.6150.280.250.0750.728t9-18:10.630.810.0750.0530.490.050.0720.7660.350.280.0620.2850.310.270.0680.576t10-18:10.840.660.1310.2180.400.350.0680.4930.400.340.0710.4140.370.290.0810.388t11-18:15.566.471.7160.6145.095.631.3980.7114.513.191.4050.3843.132.571.6330.741t12-18:10.711.030.1270.0450.871.230.1720.0830.530.410.1160.3300.420.350.1400.622t13-18:10.650.860.0720.0250.761.000.1340.1230.760.650.1350.4260.620.540.1730.641t15- + c10-18:10.590.590.0410.9990.270.350.0380.0830.490.460.0480.5850.480.440.0440.428t16- + c14-18:10.310.290.0380.7100.250.240.0330.7190.260.250.0320.6540.280.240.0470.375 c 1 8 : 1 2 6 . 1 9 2 2 . 7 9 2 . 4 4 8 0 . 2 1 4 1 1 . 5 7 1 2 . 1 2 1 . 5 7 0 0 . 7 3 7 1 5 . 8 2 1 7 . 0 3 0 . 9 5 3 0 . 2 5 3 2 2 . 5 1 2 4 . 0 1 3 . 5 6 9 0 . 6 8 9 t 1 8 : 1 9 . 3 9 1 0 . 9 6 1 . 9 6 8 0 . 4 5 3 8 . 1 6 9 . 4 5 1 . 7 4 0 0 . 4 8 6 7 . 4 1 5 . 6 6 1 . 8 3 1 0 . 3 7 7 5 . 6 7 4 . 7 6 2 . 1 8 2 0 . 6 9 2 c 9 , c 1 2 - 1 8 : 2 7 . 2 5 6 . 3 0 0 . 6 5 3 0 . 1 9 6 1 2 . 4 0 8 . 9 2 0 .7870.0042.021.700.1960.1562.151.670.1210.008c9,t12-18:2 + c16-18:10.160.220.0200.0180.050.060.0120.4390.050.050.0140.9990.070.060.0070.730t9,c12-18:20.080.160.0340.0580.080.130.0270.0800.040.030.0110.3900.050.030.0160.249t11,c15-18:20.070.190.0470.0550.030.130.0240.0080.140.080.0310.1240.170.100.0580.261t9,t12-18:20.010.020.0050.0400.010.010.0050.9960.010.000.0040.5370.020.020.0100.809c9,t11-CLAa4.554.610.7560.9391.440.910.2240.0560.370.290.1110.4860.420.360.1250.623t,t-CLA0.090.090.0170.9990.120.100.0140.1900.020.020.0050.6200.050.050.0130.72218:3n-60.030.030.0050.6200.280.140.0360.0090.030.020.0040.0970.040.020.0080.10718:3n-30.881.140.1360.1051.641.760.2530.6590.620.400.1280.1410.660.420.1380.13220:00.230.280.0550.1310.080.100.0070.0180.170.150.0240.5560.140.150.0220.66520:2n-60.040.070.0080.0050.070.080.0100.3560.020.020.0050.6200.040.030.0110.30320:3n-60.120.140.0210.3740.560.410.0470.0210.020.020.0040.5370.030.040.0050.39020:4n-60.971.000.1870.8576.095.280.8390.3700.070.060.0130.5980.100.090.0160.37620:5n-30.500.700.1110.1214.354.770.7010.5690.040.030.0070.1210.050.030.0170.337 u n s a t u r a t e d C 2 0 1 . 6 2 1 . 9 1 0 . 2 4 6 0 . 2 8 0 1 1 . 0 5 1 0 . 5 3 0 . 9 8 7 0 . 6 1 2 0 . 1 4 0 . 1 2 0 . 0 1 9 0 . 2 4 4 0 . 3 2 0 . 3 0 0 . 0 4 4 0 . 6 2 4 2 2 : 0 0 . 1 1 0 . 1 1 0 . 0 0 9 0 . 8 0 1 0 . 0 3 0 . 0 4 0 . 0 0 4 0 . 2 0 7 0 . 0 3 0 . 0 3 0 . 0 0 6 0 . 7 0 5 0 . 0 4 0 . 0 3 0 . 0 0 8 0 . 5 3 7 c 1 3 - 2 2 : 1 + 2 0 : 3 n - 3 0 . 0 3 0 . 1 1 0 . 0 1 5 0 . 0 0 2 0 . 0 4 0.090.0100.0040.030.020.010.3460.040.030.0160.56322:2n-6ndnd--0.010.030.0060.013ndnd--ndnd--22:4n-60.060.060.0040.9990.130.120.0190.3840.020.020.0050.6200.040.050.0070.20722:5n-30.941.070.1010.2524.344.590.3530.5180.170.140.0430.5500.250.250.0390.95122:6n-30.310.510.1040.0963.364.630.9420.2280.050.030.0170.2280.070.070.0190.900 u n s a t u r a t e d C 2 2 1 . 3 0 1 . 6 3 0 . 2 0 0 0 . 1 5 3 7 . 8 5 9 . 3 6 1 . 2 1 0 0 . 2 7 1 0 . 2 6 0 . 2 1 0 . 0 6 7 0 . 4 2 3 0 . 4 0 0 . 3 9 0 . 0 7 0 0 . 9 4 5 2 3 : 0 0 . 0 4 0 . 0 6 0 . 0 1 3 0 . 2 2 2 0 . 0 1 0 . 0 3 0 . 0 0 5 0 . 0 2 4 0 . 0 2 0 . 0 2 0 . 0 0 4 0 . 9 9 9 0 . 0 3 0 . 0 3 0 . 0 1 0 0 . 9 9 9 2 4 : 0 0 . 0 3 0 . 0 3 0 . 0 0 5 0 . 3 9 0 0 . 0 2 0 . 0 2 0 . 0 0 4 0 . 9 9 9 0 . 0 1 0 . 0 1 0 . 0 0 4 0 . 9 9 9 0 . 0 2 0 . 0 1 0 . 0 0 6 0 . 2 7 8 S a t u r a t e s 4 2 . 1 1 4 1 . 4 4 1 . 7 5 1 0 . 7 1 6 3 8 . 6 6 3 8 . 5 3 2 . 0 0 8 0 . 9 5 1 6 8 . 9 4 7 0 . 9 4 2 . 3 3 2 0 . 4 2 5 6 1 . 9 8 6 2 . 7 5 2 . 0 8 2 0 . 7 2 4 M o n o u n s a t u r a t e s 3 6 . 5 8 3 5 . 0 3 1 . 1 0 5 0 . 2 1 2 2 0 . 4 3 2 2 . 7 2 1 . 4 3 2 0 . 1 6 0 2 4 . 0 9 2 3 . 5 4 1 . 7 3 5 0 . 7 6 1 3 0 . 5 7 3 1 . 1 4 2 . 2 2 2 0 . 8 0 5 P o l y u n s a t u r a t e s 1 6 . 5 3 1 6 . 9 5 1 . 0 4 4 0 . 7 0 2 3 5 . 3 1 3 2 . 5 0 1 . 8 8 7 0 . 1 8 7 3 . 9 2 3 . 0 6 0 . 4 0 7 0 . 0 7 9 4 . 4 5 3 . 4 3 0 . 3 7 4 0 . 0 3 5 n - 6 p o l y u n s a t u r a t e s 8 . 4 6 7 . 6 0 0 . 7 4 6 0 . 2 9 0 1 9 . 5 3 1 4 . 9 6 1 . 0 7 7 0 . 0 0 6 2 . 1 6 1 . 8 3 0 . 1 9 7 0 . 1 3 7 2 . 4 0 1 . 8 9 0 . 1 1 6 0 . 0 0 5 n - 3 p o l y u n s a t u r a t e s 2 . 6 3 3 . 4 1 0 . 2 5 2 0 . 0 2 1 1 3 . 6 8 1 5 . 7 4 1 . 6 1 9 0 . 2 5 1 0 . 8 7 0 . 5 9 0 . 1 5 1 0 . 1 1 1 0 . 9 3 0 . 6 5 0 . 1 3 9 0 . 0 8 6 d"C 1 4 1 1 . 6 3 1 1 . 0 5 1 . 5 5 2 0 . 7 2 1 1 . 4 6 1 . 7 8 0 . 3 0 2 0 . 3 3 7 3 . 9 8 4 . 7 0 0 . 3 8 5 0 . 1 1 1 3 . 4 1 3 . 6 9 0 . 2 7 0 . 3 4 8 C 1 6 1 9 . 3 9 2 2 . 4 2 0 . 5 7 1 0 . 0 0 2 1 3 . 2 0 1 7 . 4 1 1 . 6 5 5 0 . 0 4 4 2 7 . 8 6 3 1 . 6 6 1 . 7 4 1 0 . 0 7 2 2 5 . 8 8 2 8 . 6 7 1 . 7 6 2 0 . 1 6 4 e"C 1 8 6 4 . 5 3 6 4 . 5 0 1 . 7 4 5 0 . 1 3 2 8 1 . 5 3 7 6 . 6 3 1 . 9 7 4 0 . 0 4 8 6 3 . 1 0 5 9 . 1 0 1 . 8 3 8 0 . 0 7 2 6 5 . 3 7 6 2 . 3 8 2 . 4 4 8 0 . 2 6 8 S O = s u n f l o w e r - s e e d o i l ; S F O = s u n f l o w e r - s e e d o i l a n d f i s h o i l ( 2 : 1 ) p l u s s t a r c h f r o m rolled barley: CLA = conjugated linoleic acid; nd = not detected.
a Contains c8,t10- and t7,c9-CLA as minor components.
References
Bernard L, Leroux C, Rouel J, Bonnet M and Chilliard Y 2012. Effect of level and type of starchy concentrate on tissue lipid metabolism, gene expression, and milk fatty acid secretion in Alpine goats receiving a diet rich in sunflower oil. British Journal of Nutrition 107, 1147-1159.
Bernard L, Rouel J, Leroux C, Ferlay A, Faulconnier Y and
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