ࡱ > _ bjbj <" b b ) & & & 8 ^ $ & _ : : R ^ $ # ! F E f : : f f T > > > f R > f > > > P " > / 0 _ > " d [" > " 7" $ > f f > f f f f f > f f f _ f f f f [" f f f f f f f f f : @ Supplementary Methods
2DSA GA-MC analysis
In order to estimate hydrodynamic shapes of individual species, SV data were also analyzed by two-dimensional spectrum analysis (2DSA) and then by a genetic algorithm with a Monte Carlo simulation (GA-MC) using the program UltrascanII version 9.9 ADDIN EN.CITE Demeler200575(Demeler 2005)75756B. DemelerD. J. Scott, S.E. Harding and A.J. Rowe. Eds.Modern Analytical Ultracentrifugation: Techniques and Methods.210-2292005Royal Society of Chemistry (UK)( HYPERLINK \l "_ENREF_1" \o "Demeler, 2005 #75" Demeler 2005). We performed 50 iterations. In all the analyses, time-invariant noise and radian-invariant noise were removed. Additional parameters for the analysis included partial specific volumes calculated from the amino acid composition (0.7328 cm3/g), and buffer density ( r= 1 . 0 1 2 2 g / c m 3 ) a n d v i s c o s i t y ( h= 1 . 0 1 0 1 c e n t i p o i s e s ) c a l c u l a t e d u s i n g t h e p r o g r a m U l t r a S c a n I I v e r s i o n 9 . 9 .
C D m e a s u r e m e n t o f T b I M P D H
A l l C D m e a s u r e m e n t s w e r e p e r f o r m e d u s i n g a J - 8 2 0 s p e c t r o p o l a r i m e t e r ( J A S C O , T o k y o , J a p a n ) . T h e p a t h l e n g t h s w e r e 1 m m f o r f a r - U V t o v i s i b l e r a n g e a t 2 0 0 t o 2 6 0 n m a n d 1 0 m m f o r n e a r - U V t o v i s i b l e r a n g e a t 2 5 0 t o 3 5 0 n m , r e s p e c t i v e l y . T b I M P D H c o n c e n t r a t i o n w e r e 2 mM f o r f a r - U V C D a n d 1 3 mM f o r n e a r - U V C D . T h e m e a s u r e m e n t s w e r e p e r f o r m e d a t 2 5 C i n t h e f o l l o w i n g b u f f e r s : 5 0 m M
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A D D I N E N . R E F L I S T D e m e l e r , B . ( 2 0 0 5 ) . M o d e r n A n a l y t i c 1 R S T U V c d h l m n o p ~ xnZSKGKG h) j h) Uh h; 'j hdH hdH 5CJ UmH nH uhL h; CJ o(
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h; CJ $js hdH hdH CJ UmH nH u hL ho=$ CJ o(hL h; CJ hL ho=$ CJ h; CJ aJ sH h h o(j h UhL 5mH nH u UhL hL 6mH nH u hL mH nH u al Ultracentrifugation: Techniques and Methods., Royal Society of Chemistry (UK).
Supplementary Fig. S1
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